Full-length RecE and RecT from Rac prophage mediate highly efficient linear–linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression ...

We developed an efficient "Combinatorial Strategy” for cloning different vectors with various clone sites. 1) Using originally existed clone sites from circular vectors to prepare the inserts, and if ...

The availability of effective vector systems is a pre-requisite for genetic manipulation of plants through recombinant DNA technology. We report here construction of a series of binary vectors that ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors ...

Whether it’s heat, emotions, or stress, the body sweats it out through a varied mix of volatile chemicals and microbes. A large language model can design and troubleshoot CRISPR protocols from scratch ...

Biology is no longer being hampered by the cell environment thanks to cell-free technology that makes it easier to clone DNA. Located in Tokyo, Japan, OriCiro Genomics is a pioneer in cell-free ...

Nereus Gunther is a molecular biologist at the Agricultural Research Service within the US Department of Agriculture (Washington D.C., USA), where part of his research focuses on Campylobacter and its ...