PCR Site-Directed Mutagenesis Workflow | DNASTAR

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Explain the principle of site-directed mutagenesis, then descri... | Filo

With the use of site-directed mutagenesis, hemoglobin has been prepared in which the proximal histidine residues in both the αand the βsubunits have been replaced by glycine. The imidazole ring from the histidine residue can be replaced by adding free imidazole in solution. Would you expect this modified hemoglobin to show cooperativity in oxygen binding? Why or why not? (FIGURE CANT COPY) | Numerade